Morphological and physiological approach

To further investigate adverse outcomes associated with previously selected chemicals, we will use standardized acute and chronic experimental tests with cladoseran, fathead minnow and zebrafish. Experiments will follow established US EPA guidelines. Specifically, we will measure survival, growth and reproduction rates in fish after exposure to different chemical doses. In addition to these common indicators, we will examine direct biochemical impacts of oxidative stress such as lipid peroxidation, glutathione depletion and DNA oxidation in fish using standard toxicity assays.

These studies will provide an important bridge between in vivo and in vitro common models employed for biomedical and ecological research.